Serial sectioning of teeth and microscopy in cariology research
نویسندگان
چکیده
In-vitro studies commonly establish the validity of a detection system by using a ‘‘gold standard’’ against which the diagnostic method can be evaluated. The most common gold standard used for caries lesions is the histological evaluation of hard tissue sections. In order to evaluate serial sections histologically, elaborate equipment is required. Undecalcified teeth with enamel and dentin cannot be sectioned with conventional histological procedures due to the risk of section damage. There are a number of shortcomings in histological methods, e.g. section damage during preparation of samples or the section might not relate to the actual investigation site in question. This publication presents in detail the tools, auxiliary forms and geometric procedures to embed and serially cut teeth in a three dimensional orientation, which allows accurate location of each section from within the tooth. A macro photographic reproduction technique of histological slides is also presented. This method has been applied successfully in dental education and for cariology studies.
منابع مشابه
Topics: 3D CLSM and Light Microscopy, 3D FIB/SEM or Serial Sectioning time resolved microscopy, 3D Image Analysis and simulation 3D TEM Tomography and Serial Sectioning, 3D X-ray Microscopy and Tomography, Ultra-Resolution Tomography Scientific committee and chairpersons:
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